The development of the injectable hydrogel including periosteum stem cells to increase the volume of the alveolar bone

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670849
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: UCHINO Natsuko 東京大学, 医学部附属病院, 助教 (30569637)
• Co-Investigator(Kenkyū-buntansha): KAZUMI Ohkubo 東京大学, 医学部附属病院, 講師 (10396715)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 歯槽骨再建 / 幹細胞 / ペプチドハイドロゲル

Outline of Final Research Achievements

We have focused on periosteum of mentum as an abundant source of stem cell for regeneration of oral tissues. To develop the injectable hydrogel includes the cultured stem cells based on the biocompatible self-organization peptide hydrogel ‘‘PuraMatrix’’, we have evaluated the culture method and differentiation method for maintaining pluripotency of stem cell derived from human or rat. As results, the periosteum stem cells maintained stem cell properties in comparison with cells of surrounding oral tissue. In addition, we have prepared the bone defect model and extraction socket model then implanted stem cells derived from synovium to the defect. By the animal experiments, bone regeneration was observed the abundant extracellular matrix histologically. It was clear that the promotion of bone formation was enabled by using the injectable hybrid hydrogel easily, noninvasively. In this project, it was revealed that it was an effective method for relatively a little bone defect case.

Free Research Field

口腔外科