Delivery of functional microRNA accelerates the bone regeneration with BMP2 and BMAC.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670863
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University
• Principal Investigator: ASAHINA Izumi 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039)
• Co-Investigator(Kenkyū-buntansha): SUMITA Yoshinori 長崎大学, 大学院医歯薬学総合研究科(歯学系), 准教授 (50456654) ; NISHIMURA Masahiro 鹿児島大学, 大学院医歯(薬)学総合研究科, 教授 (00294570)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 骨再生 / microRNA / BMP

Outline of Final Research Achievements

Direct implantation of freshly isolated bone-marrow aspirate concentrates (BMAC) with minimal amount of rhBMP2 to the mouse cranium were performed to assess the effectiveness of administration of functional miRNA in vivo. On the other hand, mouse bone-marrow mesenchymal stem cells (MSC) were exposed to rhBMP2 for osteoblastic induction in culture to search the candidates of miRNA. As a result, when some miRNAs, which were chosen in vitro assay, were transplanted with BMAC and minimal amount of BMP2, we found one miRNA can accelerate the new bone formation. At present, we are doing further experiments to investigate the function of this miRNA in detail.

Free Research Field

口腔外科学・再生医学