Delivery of bone-marrow stem cells and functional microRNA for atrophic salivary gland regeneration

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670864
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University
• Principal Investigator: SUMITA Yoshinori 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654)
• Co-Investigator(Kenkyū-buntansha): ASAHINA Izumi 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039) ; KAGAMI Hideaki 松本歯科大学, 歯学部, 教授 (80242866)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 萎縮唾液腺 / 細胞治療 / microRNA

Outline of Final Research Achievements

The specific hypothesis of this study is that bone marrow-derived endothelial progenitor cells (CD34-positive cells) can rescue the radiogenic salivary-gland atrophy. Furthermore, we assumed that the delivery of functional miRNA that contributes the proliferation or differentiation of salivary-gland epithelial cells may be able to enhance the effect of CD34-positive cell therapy. As a result, local administration of CD34-positive cells to submandibular glands could increase the salivary output of mice after 12Gy of head and neck irradiation. Currently, we are analyzing in vivo behavior of exogenous CD34-positive cells in detail, and administrating miRNAs with CD34-positive cells to irradiated mice.

Free Research Field

口腔外科学・再生医学