Investigation of microRNA as a biomarker in recurrence and metastasis of oral cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670867
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Hyogo Medical University
• Principal Investigator: Urade Masahiro 兵庫医科大学, 医学部, 特別招聘教授 (70104883)
• Co-Investigator(Kenkyū-buntansha): NOGUCHI Kazuma 兵庫医科大学, 医学部, 准教授 (50309473) ; ZUSHI Yusuke 兵庫医科大学, 医学部, 助教 (80581206)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 口腔癌 / マイクロRNA / 原発巣 / 転移巣 / 培養癌細胞 / 患者血清 / バイオマーカー

Outline of Final Research Achievements

The aim of this study is to examine the expression of microRNA (miRNA) that exists in oral cancer tissues and serum, and is non-coding RNA regulating expression of oncogenes and tumor suppressor genes, and to investigate the possibility to become a biomarker of oral cancer.
The microRNA expression was examined using microarray for primary tumor, metastatic tumor, cultured tumor cells (OSCC) derived from lymph node metastasis, and serum of the first visit of the same tongue cancer patient. Consequently, it was found that miR-365a-5p and miR-4756-5p were down-regulated in all samples as compared to oral keratinocyte as control, and strongly involved in growth and invasiveness of OSCC. These results indicated that these two miRs are potent biomarker of oral cancer.

Free Research Field

外科系歯学