Development of the therapeutic targets for salivary gland carcinoma using virtual pathology and antibody-based proteomics

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670868
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: National Cancer Center Japan
• Principal Investigator: HONDA Kazufumi 独立行政法人国立がん研究センター, 研究所, ユニット長 (10260936)
• Co-Investigator(Kenkyū-buntansha): MORI Taisuke 国立研究開発法人国立がん研究センター中央病院, 病理科 (00296708) ; 野村 武史 東京歯科大学, オーラルメディシン・口腔外科学講座, 教授 (60328268)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 唾液腺がん / 組織マイクロアレイ / プロテオミクス

Outline of Final Research Achievements

To identify the biomarker for decision of therapeutic strategy in salivary gland carcinoma, we made tissue microarray of the salivary gland carcinoma, and searched it by antibody-based proteomics. We identified overexpression of actiin-4 (ACTN4) that is associated with cancer metastasis in salivary gland carcinoma. To confirm the gene amplification of ACTN4, we investigated the copy number of ACTN4 by FISH analysis. FISH (fluorescence in situ hybridization) revealed that overall survival time of patients with gene amplification of ACTN4 was significantly shorter than it of patients with normal copy number. The multivariate Cox regression analysis revealed that gene amplification of ACTN4 remained as an independent prognostic factor. The gene amplification of ACTN4 is a potential prognostic biomarker for salivary gland carcinoma.

Free Research Field

バイオマーカー研究