Scleraxis-mediated regulation of mechanical responses in the periodontal ligament

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670871
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: SHUKUNAMI Chisa 広島大学, 大学院医歯薬保健学研究院, 教授 (60303905)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Teruko 東北大学, 歯学研究科(研究院), 教授 (00127250) ; TAKIMOTO Aki 京都大学, 健康長寿社会の総合医療開発ユニット, 特定助教 (00378902)
• Research Collaborator: YOSHIMOTO Yuki 広島大学, 大学院医歯薬保健学研究院, 特任助教 (40735304)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 歯学 / メカニカルストレス / 歯根膜 / Scleraxis / トランスジェニックマウス

Outline of Final Research Achievements

During experimental tooth movement, Scleraxis (Scx) and Osterix (Osx) were upregulated on the tension side of the periodontal ligament (PDL). Scx and Osx were upregulated via activation of TGF-beta and BMP signaling, respectively. Overexpression of Scx resulted in significant inhibition of mineralization in cultured PDL cells under the osteoinductive conditions. Conversely, knockdown of Scx significantly upregulated the expression of Osteocalcin, a marker for bone formation in osteoinduced PDL cells. Thus, Scx and Osx antagonistically regulate tensile-force responsive remodeling of the PDL and alveolar bone.

Free Research Field

機能系基礎歯科学