2016 Fiscal Year Final Research Report
The development of biobutanol system from plant biomass using Clostridium species
| Project/Area Number |
25701017
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | バイオブタノール / Clostridium属細菌 / 耐熱性 / メタボローム解析 / 胞子形成 / 育種 |
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| Outline of Final Research Achievements |
This study revealed that adenine addition enhances butanol production in Clostridium saccharoperbutylacetonicum cultivated at 37°C. When strain N1-4 cultivated at 37°C with the addition of adenine, the intracellular ATP level was improved. In addition, although strain N1-4 cultivated at 37°C without the addition of adenine sporulated since 6 hours, spore formation was inhibited until 24 hours with the addition of adenine at even 37°C. We isolated heat resistant mutant RIFR91 using rifampicin as an inhibitor and analyzed characteristic of the strain.
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| Free Research Field |
農芸化学・応用微生物学
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