Ultrafast optical microscopy for detection of circulating tumor cells in blood

2014 Fiscal Year Final Research Report

• Project/Area Number: 25702024
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokyo
• Principal Investigator: GODA Kisuke 東京大学, 理学(系)研究科(研究院), 教授 (70518696)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 超高速イメージング / がん / フローサイトメトリー / 循環腫瘍細胞 / 高速顕微鏡 / マイクロ流体工学

Outline of Final Research Achievements

We developed an ultrafast optical microscope for early, noninvasive, low-cost detection of cancer. This microscope is based on a combination of an ultrafast optical imager known as STEAM and a multi-color fluorescence analyzer for high-throughput screening of cells in a large heterogeneous population. Based on a Ti:Sapphire femtosecond pulse laser, we achieved high spatial resolution of 780 nm and high frame rate of 70 Mfps for high-speed morphological screening. Furthermore, we achieved focusing and ordering of cells in high-speed flow with high precision of a few micrometers. Using the developed system, we demonstrated single-cell analysis of a large population of cells with a high throughput of 10,000 cells/s. This work has been absorbed into the ImPACT program in which a far more advanced system is currently under development.

Free Research Field

光科学