Development of a stimulated Raman spectral microscope for the analysis of small biomolecules in live cells and tissues

2016 Fiscal Year Final Research Report

• Project/Area Number: 25702026
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokyo
• Principal Investigator: Ozeki Yasuyuki 東京大学, 工学(系)研究科(研究院), 准教授 (60437374)
• Research Collaborator: HASHIMOTO Hiroyuki キヤノン(株), 主席研究員 ; SAKAMOTO Michiie 慶応大学, 医学部病理学教室, 教授 ; YOKOYAMA Hiroyuki 東北大学, NICHe, 教授 ; GODA Keisuke 東京大学, 理学系研究科, 教授 ; EGAWA Mariko 資生堂, ライフサイエンス研究センター, 副主任研究員
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 誘導ラマン散乱 / 生体顕微鏡 / 生体観察

Outline of Final Research Achievements

We utilized a stimulated Raman scattering (SRS) microscope to observe living cells and tissues and to investigate the behavior of small molecules such as lipids and polysaccharides. In addition, we improved the performance of our SRS microscope and developed new laser light sources. As a result, we succeeded in visualizing lipids and polysaccharides contained in living microalgae without labeling, and discriminating between plural kinds of cells contained in skin tissues without labeling. Furthermore, we successfully demonstrated SRS imaging using a semiconductor laser diode capable of long-term stable operation and developed a new high-speed wavelength-tunable fiber laser. These results will broaden the possibility of biomedical imaging and make this technology more practical.

Free Research Field

生体医工学