Real time and reversible imaging of cytokine receptors on living cells based on supramolecular approaches

2016 Fiscal Year Final Research Report

• Project/Area Number: 25708026
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Tohoku University (2014-2016); Kyoto University (2013)
• Principal Investigator: Takaoka Yousuke 東北大学, 理学(系)研究科(研究院), 講師 (80599762)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 蛋白質間相互作用 / ケミカルバイオロジー / 超分子 / 蛋白質化学修飾

Outline of Final Research Achievements

In this project, I succeeded in development of various chemical methodologies for protein-protein interactions (PPIs), such as cytokine-receptor interaction, or antigen-antibody interaction. Moreover, I realized the identification of interaction site by using PPI-based chemical modification method on living cells, and real-time imaging of the receptors on living cells. Additionally, development of self-assembling nanoprobes for live imaging of protein activity have been achieved, and this is the first example of reversible turn-on nanoprobes inside the live cells. Finally, based on these fundamental analyses, I succeeded in development of analysis methods for phytohormone receptors which cause protein-protein interaction and regulate life cycle in planta.

Free Research Field

ケミカルバイオロジー