2015 Fiscal Year Final Research Report
Investigation of DNA replication restart mediated by MUS81-EME1 structure-specific endonuclease, RecQ helicases, and others.
| Project/Area Number |
25710010
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Research Field |
Tumor biology
|
|---|
| Research Institution | Oita University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | ゲノム不安定性 / DNA修復 / 組換え |
|---|
| Outline of Final Research Achievements |
In our daily life, various DNA stresses inhibit the progression of DNA replications. Remained unreplicated regions on chromosomes often cause chromosomal instabilities. Therefore, the repair of stalled DNA replication is crucial for maintenance of genome integrity. Previously, we have shown that MUS81-EME1 structure-specific endonuclease is involved in the restart mechanism. Here we have identified a new factor involved in the restart mechanism of DNA replication. FBH1 is the DNA helicase in which contains the F-box domain. DNA unwinding activity of FBH1 encouraged the dissociation of RAD51 from DNAs, and the F-box domain of FBH1 was required for the ubiquitin ligase activity. Indeed, FBH1 was involved in the ubiquitination of RAD51. Here we report the new functions of FBH1, contributing the restart mechanism of DNA replication.
|
| Free Research Field |
腫瘍生物学
|
