2015 Fiscal Year Final Research Report
Structural biology of thermosensation in human
| Project/Area Number |
25711004
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Tottori University |
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Principal Investigator |
Hino Tomoya 鳥取大学, 工学(系)研究科(研究院), 准教授 (40373360)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | TRPチャネル / イオンチャネル / 膜タンパク質 / X線結晶構造解析 / タンパク質精製 / タンパク質発現 / 酵母 |
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| Outline of Final Research Achievements |
Thermosensation is an essential physiological process for the proper adaptation to the changes in environmental temperature. In this study, to gain insight into the molecular mechanisms of thermosensation operated by TRP ion channels, we challenged to determine the atomic structures of human TRP channels.
We successfully overexpressed 9 thermosensitive TRP channels by employing methylotrophic yeast Pichia pastoris as a host strain and purified 5 channels by utilizing affinity column that is immobilized GFP-nanobody. In addition, we obtained several monoclonal antibodies that recognize conformational epitopes of TRPV4.
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| Free Research Field |
構造生物学
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