2015 Fiscal Year Final Research Report
Analysis of the mechanism that creates the polarity and separates germ layer fates.
| Project/Area Number |
25711016
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 細胞極性 / 胚葉運命 / 核移動 / PI3K |
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| Outline of Final Research Achievements |
I analyzed the mechanism responsible for creation of diverse types of cells that constitute the animal body from a single cell, the fertilized egg. The initial stages of this process is the formation of the germ layer cells. In previous studies, I found that mesoderm and endoderm fates are separated by nuclear migration-dependent localization of a mRNA encoding Not within the mesendoderm cell and its partitioning to the mesoderm daughter cell. In this study, I analyzed the mechanism that determines the direction of the nuclear migration, as it is central to fate separation. I found that localization of PI3K, a membrane protein, to the future mesoderm side of the mesendoderm cell determines the direction of nuclear migration. Localization of PI3K was dependent on actin dependent re-localization of egg cytoplasm immediately after fertilization. The localization was maintained by activation of PI3K in the future mesoderm region at the 4-cell stage.
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| Free Research Field |
発生生物学
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