In vitro analysis of functions of cohesin in replication-coupled sister-chromatid cohesion and DNA double-strand break repair

2016 Fiscal Year Final Research Report

• Project/Area Number: 25711022
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Kyushu University (2016); Osaka University (2013-2015)
• Principal Investigator: Takahashi Tatsuro 九州大学, 理学(系)研究科(研究院), 准教授 (50452420)
• Research Collaborator: HIGASHI Torahiko ; KAWASOE Yoshitaka ; YAMAGUCHI Yohko ; TERUI Riki ; HAYASHI Sae ; KAYUKAWA Taiki ; ODA Satomi
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: DNA複製 / 染色体接着 / 相同組換え / 二重鎖切断 / ツメガエル卵抽出液 / コヒーシン / アセチル化

Outline of Final Research Achievements

Sister-chromatid cohesion, mediated by the cohesin complex, facilitates precise segregation of sister chromosomes at M-phase and promotes repair of DNA double-strand breaks (DSBs). However, how cohesin promotes those reactions has not been clearly understood. Using Xenopus egg extracts and biochemical reconstitution with purified proteins as model systems, we revealed functional relationship between XEco2 cohesin acetyltransferase, a key enzyme in the cohesion establishment reaction, and multiple replication proteins. We also constructed in vitro model systems for post-replicative DSB repair, and analyzed involvement of cohesin in the repair reaction. This study clarified novel functional interaction between sister-chromatid cohesion and DNA replication.

Free Research Field

分子生物学