2016 Fiscal Year Final Research Report
Elucidation of mechanism for esterified carotenoids in tomato petal
| Project/Area Number |
25712004
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | トマト / カロテノイド / 変異体 / 脂肪酸 / プラストグロブリ |
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| Outline of Final Research Achievements |
This research aimed to understand molecular mechanism for esterified carotenoid using tomato mutants named pale yellow petal 1 and pyp2 that produce petals with reduced pigmentation due to reduced accumulation of the esterified carotenoid. We identified candidate genes for pyp1 and pyp2 mutants and performed complementation experiments, demonstrating that both genes were the responsible. Biochemical analyses showed that PYP1 most likely catalyzes esterification of xanthophylls with mid-chain fatty acids such as C14:0 and C16:0 as substrate. Further, plastglobule formation in plastid is highly associated with accumulation of esterified xanthophylls. Moreover, PYP1 is localized near the plastoglobule formation. These results indicated that esterified formation is essential for plastoglobule formation.
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| Free Research Field |
植物遺伝育種学
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