2016 Fiscal Year Final Research Report
Roles of the GARP complex during mammalian development
| Project/Area Number |
25712034
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kumamoto University (2014-2016)
Institute of Physical and Chemical Research (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | マウス / 初期発生 / 多能性幹細胞 / 細胞分化 / 組織発生 / 細胞内小胞輸送 |
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| Outline of Final Research Achievements |
To elucidate roles of GARP complex during mammalian development, we obtained previously established mutant mouse lines for components of the GARP complex and generated knockout mouse lines for genes that we newly identified as components of the GARP complex. From phenotypes of mutant embryos, we found that these genes could be classified into three groups. These results suggest that the GARP complex plays various roles by exchanging components during mammalian development. Furthermore, we carried out liver specific Vps52 knockout analysis for a model for identification of the role of the GARP complex during tissue development. Liver specific Vps52 knockout embryos showed very similar phenotypes to liver specific knockout embryos for an autophagy related gene. These results provide new evidence that the GARP complex seems to regulate autophagy.
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| Free Research Field |
発生遺伝学
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