Development of mitochondrial fucntional imaging using electron mediators as a probe

2015 Fiscal Year Final Research Report

• Project/Area Number: 25713004
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Kyushu University
• Principal Investigator: Hyodo Fuminori 九州大学, 学内共同利用施設等, 准教授 (10380693)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: レドックス / イメージング / ミトコンドリア / DNP / 動的核偏極

Outline of Final Research Achievements

In vivo eedox reactions that generate free radical intermediates are essential to metabolic processes on homeostasis. We report here the use of in vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) to conduct redox molecular imaging for mitochondrial functional imaging. Using in vivo DNP-MRI, we obtained simultaneous images of free radical intermediates generated from the CoQ10, FMN, and FAD involved in the mitochondrial electron transport chain, as well as the radicals derived from vitamins E and K1. Each of these free radicals was imaged in real time in a phantom comprising a mixture of free radicals localized in either lipophilic or aqueous environments. In addition, CoQH as a in vivo probe could be visualized redox alteration after mitochondrial dysfunction by KCN treatment. This study is the first to report the in vivo DNP-MRI imaging of free radical intermediates that are derived from endogenous species involved in metabolic processes.

Free Research Field

生体分析化学