Engineering radiolabeled antibody fragments for enhanced tumor to kidney ratio

2015 Fiscal Year Final Research Report

• Project/Area Number: 25713045
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Radiation science
• Research Institution: Chiba University
• Principal Investigator: Uehara Tomoya 千葉大学, 薬学研究科(研究院), 准教授 (10323403)
• Co-Investigator(Renkei-kenkyūsha): HANAOKA Hirofumi 群馬大学, 大学院医学研究科, 准教授 (50361390)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: PET / 治療 / 腎臓

Outline of Final Research Achievements

The renal localization of radiolabeled antibody fragments is a limitation of clear target imaging and radiothearpy. Herein, we describe a strategy using a 67Ga-labeling reagent for antibody fragments that liberates 67Ga-NOTA-Methionine (NOTA-Met) by the action of renal brush border enzymes to reduce renal radioactivity levels. A 67Ga-labeled Fab fragments of anti-c-kit IgG using this labeling agentl resulted in significantly lower renal radioactivity levels and clearer tumor images than those of 67Ga-labeled Fabs using conventional methods. This strategy and radiolabeling reagent would also be applicable for engineered antibody fragments and also considerable influence on the development of new diagnostic and therapeutic engineered antibody fragments.

Free Research Field

放射性薬品化学