Quantitative evaluation of radiation-induced response on tissue stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25740022
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Central Research Institute of Electric Power Industry
• Principal Investigator: Kensuke Otsuka 一般財団法人電力中央研究所, 原子力技術研究所, 主任研究員 (50371703)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 組織幹細胞 / DNA損傷 / 腸管 / 低線量放射線 / 細胞動態

Outline of Final Research Achievements

The accumulation of DNA damage in the intestinal stem cells may be crucial for the development of cancer initiating cells. Therefore, it is important to understand the kinetics of DNA repair and tissue response, which is involved in the elimination of damaged cells and tissue injury repair, in response to irradiation. In this study, DNA damage response and cellular dynamics on intestinal stem cells was determined by quantitative protocols through whole-mount single crypt immunofluorescent and flow cytometry. DNA damage repair and the elimination of damaged cells were quantified by measuring the 53BP1 and gamma-H2AX foci formation. Tissue proliferative response was evaluated by population of EGFP-positive cells and determining the EdU incorporating cells. The result indicated that radiation-induce DNA damage was repaired efficiently in Lgr5+ stem cells.

Free Research Field

放射線生物学