2015 Fiscal Year Final Research Report
Development of hypoallergenic methods of a major fish allergen, parvalbumin
| Project/Area Number |
25750023
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | パルブアルブミン / 加熱 / IgE / 変性 / 立体構造 |
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| Outline of Final Research Achievements |
It has been believed that allergenicity of a major fish allergen, parvalbumin (PV) is highly heat-stable. However, we revealed that IgE-binding sites, which are important for eliciting of allergies, exist on a conformation of PV, and it was predicted a possibility of reduction of allergenicity by denaturation of PV using heat treatment. Although heating load, such as usual cooking, did not reduced allergenicity of PV, Heavy heating load, e.g. at 100℃ for 320 min, at 120℃ for 60 min, and at 140℃ for 10 min, completely reduced allergenicity of PV. In addition, we revealed that heat treatment generated collapse of the conformational structure of PV. Furthermore, we elucidated that high heating loads can reduce allergenicities of PV from various fish species.
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| Free Research Field |
アレルギー学
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