Mechanism elucidation of Vibrio parahaemolyticus-mediated inflammation targeted for intestinal epithelial cell.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25750043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: The University of Tokushima
• Principal Investigator: SHIMOHATA Takaaki 徳島大学, ヘルスバイオサイエンス研究部, 助教 (90609687)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 腸炎ビブリオ / 腸炎 / ３型分泌機構

Outline of Final Research Achievements

In order to identify the mechanism of Vibrio parahaemolyticus-induces intestinal inflammation,　we tried to investigate 3 experiments, (1) search for new binding element of VP1680 in host cells, (2) assessment of intestinal inflammation with mice animal model, and (3) the modulation of VP1680 and T3SS1 associated gene in infection model.
In (1) search for new binding element of VP1680 in host cells, we found two VP1680 binding proteins in host cells. And (2) assessment of intestinal inflammation with mice animal model, we confirmed neutrophil migration into mice intestine. Finally, in study of (3) the modulation of VP1680 and T3SS1 associated gene in infection model, T3SS associated gene and VP1680 mRNA expression level were decreased by blockade of bacterial attachment for host cells with Transwell system. Our data may serve good understanding in VP1680-induces inflammation.

Free Research Field

食品衛生学