2014 Fiscal Year Final Research Report
Quantitative analysis of the absorption and metabolism of purines in order to control the uric acid levels
| Project/Area Number |
25750055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 尿酸値 / HPLC / プリン体 / 食品 |
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| Outline of Final Research Achievements |
To evaluate cellular uptake and purine transport, we developed a high-performance liquid chromatography method for intra- and extracellular purine quantification. The developed HPLC method was successfully applied to the qualitative analysis of 22 different intra- and extracellular purines, demonstrating that it is useful for studying the overall pattern of purine metabolism. This method could also be useful for evaluating metabolic dynamics of purines under a variety of conditions stimulated to culture cells. We used the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability and the HepG2 cells for human hepatic cells. As a result of evaluating the risk of rising uric acid levels by food ingredients, the contribution of the purine metabolism was observed rather than the intestinal permeability of the purine.
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| Free Research Field |
分析化学
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