2014 Fiscal Year Final Research Report
Development of DNA marker for melon seed remains and the applilcation for Japanese melon
| Project/Area Number |
25750102
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cultural assets study and museology
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 作物育種 / 遺伝的多様性 / ウリ / 考古学 / 選抜 / 種子遺存体 / DNAマーカー / メロン |
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| Outline of Final Research Achievements |
To develop DNA marker for seed remains excavated at archaeological site, we found a total of 102 DNA polymorphisms after sequencing the chloroplast and nuclear genome in melon. Of them, 28 sequence polymorphisms were converted to DNA marker. Phylogenetic analysis using the DNA marker presented following results: genetic differentiation between Euro-American and East Asian melons, large genetic diversity in South Asian melon including landrace, and melon introduction to Japan. Application of the DNA marker for DNA analysis in melon seed remains excavated at the Shikata archaeological site in Okayama Prefecture indicated utilization, introduction of diversified melon from the Yayoi to Edo Period and selections in respective periods as well as modern, and showed usefulness of the DNA maker for evaluating genetic diversity in Japanese crop. The DNA marker will be applied for the future study to concern social and political condition, and preference, which associate with crop selection.
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| Free Research Field |
植物細胞遺伝学
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