2014 Fiscal Year Annual Research Report
What is the molecular mechanism of stress fiber disassembly caused by cyclic stretch?
| Project/Area Number |
25750154
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
黄 文敬 九州工業大学, 若手研究者フロンティア研究アカデミー, 研究員 (00633413)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | Cyclic stretch / stress fiber disassembly / lengthening phase / shortening phase / real-time observation |
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| Outline of Annual Research Achievements |
Based on a novel hypothesis, the research aimed to figure out the molecular mechanisms of stress fiber disassembly in non-muscle cells exposed to cyclic stretch. I have specified the molecule mechanisms responsible for the disassembly of stress fibers by enhancing or suppressing the activities of cofilin or non-muscle myosin II and pointed out that the effect of non-muscle myosin II was more essential for stress fiber disassembly.
Seeing is believing. In 2014, in order to further establish the validity of this conclusion, I tried to figure out the patterns (intact sarcomeric units or G-actin monomers) of stress fiber disassembly. Since conventional cyclic stretch apparatus are not suitable for real-time observation, a new cyclic stretch system was developed to confirm the effects of lengthening/shortening phases of cyclic stretch on stress fiber disassembly. Because the displacements of stretch chamber in horizontal and vertical directions were compensated in real-time, changes of stress fibers caused by mechanical stimulation can be observed during the lengthening/shortening phase of each cycle using a camera mounted on a microscope, without turning off the apparatus, and therefore, investigation of specific effects of lengthening/shortening phases and the patterns of stress fiber disassembly became possible.
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[Journal Article] Sensitivity and Dose Denpendency of Radiation-induced injury in hematopoietic Stem/Progenitor Cells in Mice2015
Author(s)
Chang-Ying Guo, Lan Luo, Yoshishige Urata, Shinji Goto, Wen-Jing Huang, Syu Takamura, Fumiko Hayashi, Hanako Doi, Yuriko Kitajima, Yusuke Ono, Tomoo Ogi, and Tao-Sheng Li
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Journal Title
Sci. Rep.
Volume: 5
Pages: 8055
DOI
Peer Reviewed / Open Access
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