Reconstruction of high-density tissues utilizing complex cellular aggregates and microfabricated hyrdogels

2014 Fiscal Year Final Research Report

• Project/Area Number: 25750171
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Chiba University
• Principal Investigator: YAMADA Masumi 千葉大学, 工学(系)研究科(研究院), 准教授 (30546784)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ハイドロゲル / 生体組織工学 / 血管 / 肝臓 / マイクロ流体デバイス

Outline of Final Research Achievements

We have developed new strategies for reconstructing three-dimensional tissues in vitro, which are composed of densely packed cells with precise alignments. In order to achieve this, we proposed a process to pile up non-spherical unit cellular aggregates composed of multiple types of cells. First, anisotropic hydrogel microfibers or non-cell-adhesive microchambers were used to prepare linear organoids and anisotropic toroidal aggregates. Then, by organizing these unit structures within perfusion chambers or channels, we prepared high-density cellular tissues incorporating vascular networks and thick blood vessel models. In addition, we evaluated the effects of perfusion culture conditions on the functions of liver and blood tissues.

Free Research Field

生物化学工学