Functionalization and DDS application of extracellular vesicles

2014 Fiscal Year Final Research Report

• Project/Area Number: 25750173
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto University
• Principal Investigator: SAWADA Shin-ichi 京都大学, 工学(系)研究科(研究院), 助教 (50444104)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: エクストラセルラーベシクル / エクソソーム / 両親媒性高分子 / 疎水化多糖 / カチオン性分子

Outline of Final Research Achievements

Extracellular Vesicles (EVs) such as exosome are nanovesicles secreated by various types of cells. EVs are known to include proteins (cytosolic and membrane proteins) and RNAs. EVs are considered as proteins and RNAs (mRNA and miRNA) cargo for cell-cell communication, and there is an exponentially growing interest in medical application of exosomes. We propose new strategy for application of exosomes to DDS by modifying the surface of EVs with functional polymers. We developed the modification of EVs surfaces by amphiphilic polysaccharide nanogels and cationic nanogels. Moreover, interactions of EVs and cationic nanogels modified EVs to cells were investigated. The cell did not uptake well the exosomes alone. The cationic nanogels modified EVs, however, were significantly interacted with cells. Functionalization of EVs with nanogels is promising for wide application of exosomes.

Free Research Field

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