2015 Fiscal Year Final Research Report
Usefulness of noninvasive monitoring method for tissue-specific energy metabolism in human
| Project/Area Number |
25750334
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Sports science
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 骨格筋 / エネルギー代謝 / 運動 / アミノ酸 |
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| Outline of Final Research Achievements |
This study examined noninvasive metabolic markers in serum, urine, and saliva by LC-MS/MS system to evaluate energy productive balance among nutrients during and after endurance exercise in human. Immediately after marathon race, high sensitive method could measure the elevations of lactate, acetyl-carnitine as end-production of fatty acid beta-oxidation, 3-hydroxybutyrate (3HB) as ketone body, 3-hydoroxyisobutyrate (3HIB) and 3-hydroxy-3-methylbutyrate as intermediates of valine and leucine catabolism, and N-acetyltaurine (NAT) as a metabolite of acetate in serum. After a transient endurance running, urine NAT excretion was also observed over time. In addition, this method could observe the real-time energy metabolism of lipid and amino acid during and after the endurance exercise by measuring of 3HB and 3HIB in saliva. The results confirmed that the high-sensitive detective method was useful for noninvasive and real-time monitoring of tissue-specific energy metabolism in human.
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| Free Research Field |
病態栄養学
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