2015 Fiscal Year Final Research Report
Investigation the mechanism of hypoglycemic state induced atherogenic progression
| Project/Area Number |
25750361
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 低血糖症 / 新生内膜形成 / アドレナリン作用 |
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| Outline of Final Research Achievements |
In this study,insulin was injected intraperitoneally into nonobese diabetic Goto-Kakizaki (GK) rats, once every3 days for 4 weeks after balloon injury of carotid artery to induce hypoglycemia. Then, we evaluated balloon injury-induced neointima formation. Insulin treatment enhanced neointima formation and increased the number of proliferating cell nuclear antigen (PCNA)-positive cells in the carotid artery. Injection of glucose with insulin prevented hypoglycemia and abrogated intimal thickening. Also, bunazosin, an α1 adrenergic receptor antagonist, prevented intimal thickening and accumulation of PCNA-positive cells induced by insulin treatment despite the presence of concomitant hypoglycemia and high adrenaline levels. Incubation of cultured smooth muscle cells with adrenaline resulted in a significant increase in their proliferation and G0/G1 to S phase progression These adrenaline-induced effects were abrogated by bunazosin.
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| Free Research Field |
代謝内分泌学 （糖尿病学・内分泌学）
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