2014 Fiscal Year Final Research Report
Structral analysis of amphotericin B channel complex using solid-state NMR
| Project/Area Number |
25750383
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
UMEGAWA Yuichi 大阪大学, 理学(系)研究科(研究院), 助教 (20587779)
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| Research Collaborator |
MURATA Michio 大阪大学, 理学研究科, 教授 (40183652)
MATSUMORI Nobuaki 九州大学, 理学研究院, 教授 (50314357)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | アンフォテリシンB / リン脂質二重膜 / 固体NMR |
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| Outline of Final Research Achievements |
Sterol derivatives which have different side-chain or alicyclic structures were synthesized, and their affinities to amphotericin B (AmB) were evaluated by using ion-flux assay. The results indicated that face-face Van der Waals interactions stabilized the AmB-ergosterol complex. Solid-state NMR using fluorine 19 or carbon 13 labeled AmB and/or ergosterol were examined to obtain intermolecular distance information.
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| Free Research Field |
天然物化学
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