2014 Fiscal Year Final Research Report
The mechanism of action of small molecule that induces monopolar spindle
| Project/Area Number |
25750389
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 活性発現の分子機構 / 標的タンパク質 / 細胞分裂 |
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| Outline of Final Research Achievements |
Pyrenocine A induces monopolar spindle against HeLa cells. To reveal the mechanism of action, we analyzed the localization of pyrenocine A in a cell using pyrenocine A-alkyne derivative. As a result, the derivative was found to localize to centrosome, which suggested that pyrenocine A bound to proteins at centrosome. To identify target proteins of pyrenocine A, we purified pyrenocine A-binding proteins using pyrenocine A-alkyne derivatives. The obtained binding proteins were found to be enolase 1 and elongation factor 1-alpha 1 by LCMS/MS analysis.
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| Free Research Field |
ケミカルバイオロジー
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