2015 Fiscal Year Final Research Report
Construction of a guide-RNA for site-directed RNA mutagenesis utilizing intracellular A-to-I RNA editing
| Project/Area Number |
25750390
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | RNA編集 / ガイドRNA / RNA変異導入 / in vitroセレクション / 分子設計 |
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| Outline of Final Research Achievements |
Genetic engineering technologies for regulating intracellular target-gene function and/or expression have been widely used for basic research of vital biological phenomena. RNA mutagenesis has the potential to become a powerful gene-regulation methodology as an alternative to DNA mutagenesis. Adenosine-to-inosine (A-to-I) RNA editing that alters genetic information at the transcript level is an important biological process widely conserved in metazoans. Therefore, a versatile RNA mutagenesis method can be developed by taking advantage of intracellular the RNA editing mechanism. Here, we developed a novel guide RNA that is capable of inducing A-to-I mutations by guiding the editing enzyme, human adenosine deaminase acting on RNA.
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| Free Research Field |
生体分子化学
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