2015 Fiscal Year Final Research Report
Modulation of pre-miRNA processing by a small-molecule
Project/Area Number |
25750393
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Chemical biology
|
Research Institution | Osaka University |
Principal Investigator |
Murata Asako 大阪大学, 産業科学研究所, 助教 (50557121)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | マイクロRNA / 小分子化合物 / 核酸 / 発現制御 / バイオテクロノジー |
Outline of Final Research Achievements |
This study aimed to modulate the cleavage of pre-miRNAs (miRNA precursors) by a small-molecule compound. Small-molecule compounds that bind to a specific sequence in the pre-miRNA have been used to alter the cleavage reaction with Dicer, and thereby increase/decrease the production of the miRNA. Terminal loop sequences of pre-miRNA that bind to our newly designed naphthyridine derivative RND were obtained by in vitro selection from a pre-miR-29a mutant library with the randomized terminal loop region. Binding of RND to the obtained terminal sequences significantly interfere with the cleavage of the terminal loop mutants of pre-miR-29a. Gel shift analysis suggested that the inhibitory effect of RND might be caused by the formation of loop-loop complex between two terminal loop mutants upon binding of RND to the terminal loop.
|
Free Research Field |
ケミカルバイオロジー
|