Understanding the neurovascular coupling mechanisms and its neural effects

2014 Fiscal Year Final Research Report

• Project/Area Number: 25750400
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Basic / Social brain science
• Research Institution: The University of Electro-Communications
• Principal Investigator: MASAMOTO KAZUTO 電気通信大学, 情報理工学(系)研究科, 准教授 (60455384)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 神経血管連関 / グリア / 蛍光グルコース誘導体 / レーザースペックル血流計

Outline of Final Research Achievements

We developed an in vivo imaging method for glucose transportation from blood circulation to brain cells using fluorescent glucose analogue and two-photon microscopy in the awake and anesthetized rodents. The fluorescent glucose was intravenously injected to the animals, while the three-dimensional fluorescent images of the brain cells and vasculatures were captured up to depths of 0.3 mm in the cortex. We found that the fluorescent glucose was slowly transferred to the tissue, and this transportation pathway differs between the awake and anesthetized animals. Our results indicate that the isoflurane anesthesia may open the blood-brain barrier, which enhance the tissue leakage of the intravenously-injected fluorescent glucose.

Free Research Field

脳計測科学