A study on optical imaging of spreading depolarization and its photobiomodulation in central nervous system disease

2014 Fiscal Year Final Research Report

• Project/Area Number: 25750404
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Basic / Social brain science
• Research Institution: National Defense Medical College
• Principal Investigator: KAWAUCHI Satoko 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 防衛医学研究センター, 助教 (20506505)
• Research Collaborator: SATO Shunichi 防衛医科大学校, 防衛医学研究センター・情報システム研究部門, 准教授 (90502906)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 脳梗塞 / 光イメージング / 梗塞周辺脱分極 / 拡延性脱分極 / 光散乱 / 低出力光治療

Outline of Final Research Achievements

To understand the pathophysiology and to develop a new therapeutic technique for ischemic stroke, we performed near-infrared (NIR) diffuse reflectance imaging for a rat stroke model and examined whether an infarct core and periinfarct depolarization (PID) can be visualized by detecting light-scattering signal. After middle cerebral artery occlusion, light scattering increased in the ischemic core, around which a wave-like scattering change was generated and spread over the cortex repetitively, indicating occurrence of PIDs. The region with increased scattering in the ischemic core was thought to reflect cell morphological change associated with infarction. We also performed 808-nm low-intensity NIR laser irradiation to the rat brain and observed that both total number of the occurrence of PIDs and infarct region tended to decrease. These results might be caused by an increase in mitochondrial energy production by 808-nm NIR laser irradiation.

Free Research Field

医用生体光学