Label-free exosome counting for early diagnosis of cancer

2014 Fiscal Year Final Research Report

• Project/Area Number: 25790028
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nanobioscience
• Research Institution: Nagoya University
• Principal Investigator: YASUI Takao 名古屋大学, 工学(系)研究科(研究院), 助教 (00630584)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: エクソソーム / 非標識カウンティング / microRNA / がん早期診断

Outline of Final Research Achievements

In this research, we developed a label-free counting device for exosomes using diffracted light derived from nanospaces. For the detection of exosomes with 100 nm in diameter, we fabricated nanospaces (150 nm in width and height), or nanostructures (10-100 nm in diameter) embedded in microchannels. After that, we introduced biological samples (with and without exosomes), such as plasma, urine, and cell supernatant, into the microchannels, and we compared label-free signals. Finally, we could achieve highly sensitive label-free detection of exosomes.

Free Research Field

ナノバイオテクノロジー