Hydrogel-based immunocapture sheet for in vitro evaluation of contraction-dependent myokine secretion in skeletal muscle cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25790031
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Tohoku University
• Principal Investigator: NAGAMINE Kuniaki 東北大学, 工学(系)研究科(研究院), 助教 (00551540)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ハイドロゲル / 骨格筋 / マイオカイン

Outline of Final Research Achievements

Hydrogel-based immunocapture sheet for in situ detection of myokines secreted from contracting skeletal muscle cell was developed to examine exercise-dependent metabolic regulation. For stable lamination of the immunocapture sheet on wet cell or tissue surface, fibrin-based biocompatible glue was also examined, and showed stable bonding of wet cationic and anionic hydrogels. The soft, molecularly permeable hydrogel-based immunocapture sheet was directly laminated to the skeletal muscle cells and sidplayed usefully measureable changes in myokine secretion of the muscle cells upon electrically-induced contraction. This hydrogel-based bioassay sheet will allow in vitro study of myokine-dependent regulation of glycemic homeostasis in the body, and will help us to understand the mechanism of exercise therapy for type-2 diabetes.

Free Research Field

細胞電気化学