2014 Fiscal Year Final Research Report
Toward Developing a Microdevice for Elucidating the Mechanism of Blood-Nerve Interactions
| Project/Area Number |
25790034
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Nano/Microsystems
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
WASHIZU Masao 東京大学, 工学系研究科, 教授 (10201162)
OANA Hidehiro 東京大学, 工学系研究科, 准教授 (20314172)
YANARU Rina 東京大学, 工学系研究科
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 細胞接着制御 / メッシュ培養法 / 細胞シート / 血管ー神経モデル / 共培養システム / 物質輸送計測 / マイクロデバイス |
|---|
| Outline of Final Research Achievements |
Cell-cell interactions and cross-boundary transport of essential substances are important for the functioning of most body tissues. In order to measure cell-cell interaction and transport at the blood-nerve interface, we developed a novel technology for fabricating free standing cell sheets where cells are cultured on microfabricated mesh sheets set suspended in a culture medium. The mesh sheets have narrow mesh strands (3-5 μm in width) for cell adhesion and large apertures (100-200 μm in pitch) such that when suspended, cells cultured on them grow under the condition of highly minimized adhesion area. Minimization of cell-substrate interaction results in an increase in cell-cell adhesion and induces self-assembly organization of cells into cell sheets. We are currently using this method to develop cell sheets of epithelial and endothelial blood cells that will be stacked to create a blood vessel model for eventual integration with nerve cells to realize a blood-nerve system model.
|
| Free Research Field |
マイクロバイオシステム工学
|
