Fluorescence Cell Imaging Using Silica Nanoparticles Modified Chemically with Rare Earth Metal Complexes Emitting in Near-Infrared Region

2015 Fiscal Year Final Research Report

• Project/Area Number: 25810086
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Wakayama University
• Principal Investigator: NAKAHARA Yoshio 和歌山大学, システム工学部, 准教授 (10432600)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 希土類金属錯体 / 近赤外蛍光 / シリカナノ粒子 / シッフ塩基 / 逆ミセル / 水分散 / 蛍光寿命 / 細胞イメージング

Outline of Final Research Achievements

A fluorescent silylated rare earth metal complex was prepared by a very simple procedure through formation of a Schiff base between a rare earth metal complex bearing a formyl group and 3-aminopropyltriethoxysilane. Using the silylated rare earth metal complex, silica nanoparticles modified chemically with rare earth metal complexes (R-SNPs) were synthesized by a reverse micelle method with Triton X-100 as a surfactant in cyclohexane. The fluorescence properties of rare earth metal complexes immobilized in silica nanoparticles were remarkably improved by photostability, pH dependence and fluorescence lifetime, compared to free rare earth metal complexes. Also, R-SNPs showed excellent dispersibility in aqueous solution and they hardly aggregated even under pseudo-psychological conditions. Furthermore, R-SNPs was usable as a label agent for the fluorescent imaging of African green monkey kidney cells.

Free Research Field

分析化学