2015 Fiscal Year Final Research Report
Simultaneous measurment for ability of tumour-cell invasion and degradation of collegen IV on a surface of single living cell
| Project/Area Number |
25810087
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
Shoji Atsushi 東京薬科大学, 薬学部, 講師 (90459850)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | コラーゲン IV / カテプシン B / バイオセンサー / 表面プラズモン共鳴 |
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| Outline of Final Research Achievements |
We have developed a method of real-time measuring cathepsin B activity for degrading collagen IV, which is a fundamental component of the extra cellular matorix proteins. This method has indicated that collagen IV was degraded by exopeptidase and endopeptidase activity. In addition, an evaluation method of inihibitory activity for digesting collagen IV has been developed. In order to develop an imaging method for degradation of collagen IV in local area formed between cell and collagen IV, we have made a surface plasmon resonance imaging apparatus.
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| Free Research Field |
生体分析化学
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