2014 Fiscal Year Final Research Report
Development of multicolor fluorescent probes for the detection of nucleic acids using GFP chromophores
| Project/Area Number |
25810094
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 蛍光核酸 / 三重鎖DNA / RNA / 緑色蛍光タンパク質 / GFP |
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| Outline of Final Research Achievements |
In this study, we tried to develop multicolor fluorescent nucleic acids probes for the simultaneous detection of a number of functional RNAs. In order to effectively develop multicolor fluorescent probes, we focused on GFP fluorophores. Since several GFP fluorophores derivatives are already known , so that we expected using these fluorophores makes development of multicolor fluorescent probes much easier. To develop fluorescent turn on probes, we used our previous fluorescent turn on system based on DNA triplex formation.
we achieved synthesis of several fluorescent nucleic acids probes that show fluorescence upon binding to target nucleic acids.
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| Free Research Field |
生物有機化学
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