Development of the directed evolution methods for the improvement of [NiFe]-hydrogenase

2016 Fiscal Year Final Research Report

• Project/Area Number: 25810095
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Shinshu University
• Principal Investigator: IHARA Masaki 信州大学, 学術研究院農学系, 助教 (50391868)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 蛋白質工学 / ヒドロゲナーゼ / 水素 / 酸素耐性

Outline of Final Research Achievements

One of the results we achieved in this study is to establish the easy-to-use host-vector system for the production of the active [NiFe]-hydrogenase. So far, the production of the [NiFe]-hydrogenase mutants was time-consuming and labor-intensive work because the conventional host-vector systems are not available due to the complicated post-translation process for the maturation of the enzyme. We demonstrated for the first time that the balance of the expression level of the structural [NiFe]-hydrogenase genes and those responsible to post-translation process is critical for the completion of the post-translation process. We successfully established the novel host-vector system by regulating the those expression level and the [NiFe]-hydrogenase random library.

Free Research Field

蛋白質工学