Development of functional soft matter based on dameged DNA.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25810102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Kyushu University
• Principal Investigator: Toshikazu Ono 九州大学, 工学(系)研究科(研究院), 助教 (20643513)
• Co-Investigator(Renkei-kenkyūsha): HISAEDA Yoshio 九州大学, 大学院工学研究院, 教授 (79777251)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ナノ材料 / エキシマー / エキシプレックス / センサー

Outline of Final Research Achievements

We report the development of simple fluorogenic probes that report on the activity of uracil DNA glycosylase (UDG) enzymes. The probes are built from short, modified single-stranded oligonucleotides containing natural and unnatural bases. The combination of multiple fluorescent pyrene and/or quinacridone nucleobases yields fluorescence at 480 and 540 nm (excitation 340 nm), with large Stokes shifts of 140 to 200 nm. They are strongly quenched by uracil bases incorporated into the sequence, and they yield light-up signals of up to 40-fold, or ratiometric signals with ratio changes of 82-fold, on enzymatic removal of these quenching uracils. The use of similar concepts, we report the development of simple fluorogenic probes that report on the activity of 8-oxoguanine glycosylase.

Free Research Field

高分子化学、超分子化学、ケミカルバイオロジー、結晶工学