Analyzing cellular bandpass filtering with real-time traction force microscopy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25820017
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Materials/Mechanics of materials
• Research Institution: 防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群)
• Principal Investigator: Tsukamoto Akira 防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工, その他部局等, 講師 (90511460)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 培養細胞 / 引張刺激 / 細胞牽引力 / リアルタイム観察

Outline of Final Research Achievements

PDMS chamber was coated with low-elasticity PDMS to realize traction force microscopy in cells under mechanical stretching. In the low-elasticity PDMS, fluorescence beads were dispersed sufficiently by loading mechanical shearing. Mechanical stretching induce large displacement which make traction force microscopy difficult to quantify. To adjust the displacement, fluorescence images were correlated with fluorescence beads on which cells were not present. Although real-time traction force microscopy in cells under mechanical stretching is under construction, real-time imaging of intracellular Ca2+ increase in cells under mechanical stretching was demonstrated.

Free Research Field

細胞バイオメカニクス