Expansion of the central carbon metabolism in Saccharomyces cerevisiae

2014 Fiscal Year Final Research Report

• Project/Area Number: 25820400
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: MATSUDA Fumio 大阪大学, 情報科学研究科, 准教授 (50462734)
• Co-Investigator(Renkei-kenkyūsha): ISHII Jun (40512546)
• Research Collaborator: MORITA Keisuke ; NOMURA Yuta
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: バイオプロダクション / 出芽酵母 / 代謝シミュレーション / 中心代謝経路

Outline of Final Research Achievements

The central carbon metabolism in baker's yeast, Saccharomyces cerevisiae, was expanded by introducing metabolic pathways of other microorganisms to improve a capability of the yeast metabolism for more efficient bioproduction of useful chemicals. The metabolically engineered S. cerevisiae strains expressing bacterial Entner-Doudrouf and phosphoenolpyruvate carboxylase pathways were constructed whose functions were confirmed by the labeling experiment and isobutanol fermentaion test.

Free Research Field

代謝情報工学