Assessment of deviation from the undifferentiated state in colonies of hiPSCs and development of its maintenance culture process

2014 Fiscal Year Final Research Report

• Project/Area Number: 25820401
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: KIM MEE-HAE 大阪大学, 工学(系)研究科(研究院), 助教 (30506449)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: バイオ生産プロセス / 再生医療 / ヒトiPS細胞 / 未分化維持

Outline of Final Research Achievements

In this study, we describe an understanding of the possible mechanisms that control hiPSC self-renewal in cell culture conditions in the presence of feeder cells. From the standpoint of the deviation from the undifferentiated state in colonies of hiPSCs on feeder layers, the most important aspects for cell migration and growth inside a single colony are a balance between cell-cell and cell-substrate interactions that induces spatial differences in their morphological and functional characteristics. This approach can be applied to an important generic technology for expanding the in the undifferentiated state of hiPSCs.

Free Research Field

工学