2015 Fiscal Year Final Research Report
Construction of protein expression system using the desiccation tolerance peptide and the elucidation of the mechanism
Project/Area Number |
25820405
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biofunction/Bioprocess
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Research Institution | Kyushu Institute of Technology |
Principal Investigator |
Ikeno Shinya 九州工業大学, 大学院生命体工学研究科, 准教授 (20437792)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | LEAペプチド / 共発現 / 大腸菌 / タンパク質発現 |
Outline of Final Research Achievements |
We have designed LEA peptide based on the motif sequence of LEA protein from polypedilum vanderplanki, and the protein expression system was constructed to co-express target protein with the LEA peptide in E.coli. The purpose of the study is to construct and characterization of LEA peptide 13mer motif for the expression of target protein in E.coli, through the co-expression system. In this study we employed the mutation in our original LEA peptide sequence and replace the Glycine with other amino acids at the 6th and 12th position. The protein expression increased with time in LEA-K and LEA-N was co-expressed compared with co-expression with original LEA peptide. In contrast, the expression of GFP was reduced in LEA-S as compared to original one. We have also investigated enhancement of protein expression using LEA peptide co-expression system to control expression levels of the peptide. The expression of a target protein was more than ten times to co-express with the LEA peptide.
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Free Research Field |
生物工学
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