The analysis of the mitophagy in Parkinson's disease with new autophagy probes.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25830043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Katayama Hiroyuki 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (00415126)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: オートファジー / 蛍光タンパク質 / マイトファジー / パーキンソン病

Outline of Final Research Achievements

In this study, I have produced new autophagy and mitophagy probes with the two fluorescent proteins. These were able to detect autophagy and mitophagy in mammalian cells, respectively. The signals of those remained after fixation. so, those are suitable for observing with fixed samples. Actually, this autophagy probe was able to detect the starvation induced autophagy signal in mouse liver sections prepared using perfusion fixation.
These new probes would be good tools for autophagy and mitophagy research.

Free Research Field

細胞生物学