2014 Fiscal Year Final Research Report
Multiple analysis of intracellular responases in intractable disease related to dopaminergic neuron vulnerability with the use of Parkinson's Disease specific iPS cells
| Project/Area Number |
25830055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Hoshi University (2014)
Keio University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | iPS 細胞 / パーキンソン病 / メタボローム |
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| Outline of Final Research Achievements |
In the present study, iPSCs were generated from two Parkinson’s patients and two control subjects. All of the clones differentiated into neurons included tyrosine hydroxylase-positive neurons. Under the present condition, I found differences in gene expression between control and patients. Among those, the expression level of PGC-1α, which plays a key role in mitochondrial biogenesis and energy metabolism, in Parkinson’s specific-iPS cell (PD-iPSCs)-derived dopaminergic (DA) neurons was significantly lower than that in control. Using CE-MS-system, I found the change in the expression of several metabolites in glycolysis and glutathione metabolism pathways in DA neuron-derived from PD-iPSCs. Interestingly, the expression of S-adenosylmethionone, which can lead to methylation of DNA, was significantly increased in PD-iPSCs-derived DA neurons. These findings suggest that metabolic abnormality in DA neurons could lead to neuronal dysfunction in PD.
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| Free Research Field |
神経科学
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