2014 Fiscal Year Final Research Report
Generation of transgenic pigs expressing Fluorescent Ubiquitination-based Cell Cycle Indicator, Fucci.
| Project/Area Number |
25830066
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Meiji University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | Fucci / 細胞周期 / 蛍光プローブ / ブタ / 体細胞核移植 |
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| Outline of Final Research Achievements |
This study aimed to create genetically modified pigs expressing Fucci, a fluorescent probe that allows the real-time visualization of the cell cycle in living cells, by using somatic cell nuclear transfer (SCNT). Porcine fetal fibroblasts introducing Fucci vector were successfully established as nuclear donor cell for SCNT. Cloned pigs were subsequently generated using the cell established through SCNT and their progeny were created by sexual reproduction. Fluorescence observation of the tissues and organs of the cloned pigs and their progenies did not confirm the systemic expression of Fucci, but confirmed Fucci expression in some of the skin, the heart and the skeletal muscles. These results demonstrated that transgene silencing occurred following SCNT. DNA methylation analysis revealed that the promoter sequences in Fucci transgene was highly methylated.
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| Free Research Field |
実験動物学
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