2014 Fiscal Year Final Research Report
Development of recurrence mouse brain tumor model using inducible iCSCs and identification of target molecules in recurrence tumor
| Project/Area Number |
25830088
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Tumor biology
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 神経幹細胞 / ＡＬＫキナーゼ / 脳腫瘍モデル |
|---|
| Outline of Final Research Achievements |
The cancer stem cells (CSCs) are thought that the cause of local recurrence or distant organ metastasis following therapy. In this study, we have established the inducible mouse brain tumor model transplanting the genetically modified neural stem cells (NSCs) using Tet-On system. Inducible expression of activated-ALK could transform the NSCs in the presence of Dox treatment in mice. Next, we performed the exome sequence analysis to investigate the genetic aberration in the activated-ALK or -H-RAS expressing NSCs. Exome data showed that characteristic change of copy number in each chromosomes were observed in these cells. Using the inducible mouse brain tumor model, we would like to establish the recurrence mouse brain tumor model and identify the target molecules in recurrence tumors by analyzing copy number change.
|
| Free Research Field |
腫瘍生物学
|
